Unequivocal data is now available to confirm 'exactly' how many platelets you really harvest with PRP, and why i-PRF is the next generation and superior, more effective option when it comes to autologous treatments in regenerative therapy.
Introduction In the last few decades different concepts have evolved for the best possible tissue regeneration in wounds. However the lack of standardisation and scientific data to prove exactly 'how many platelets' are harvested following centrifugation has been sparse.... UNTIL NOW!!
Method Four normal adults each donated a total of 141 mL of whole blood, some of which served as a control and the remainder of which was processed through three different PRP and one i-PRF separation kits. The resultant PRP was tested for platelet count, red blood cell count, and white blood cell count, including differential in a commercial pathology laboratory. Quantitative research was the best approach with the aim to use computational techniques to extrapolate numerical data accurately and then compare the numerical results. A comparative analysis was undertaken to determine:
a) The baseline of a normal cell platelet counts within blood without being centrifuged – control sample
b) The platelet counts (concentration) of each kit after they have been centrifuged according to their manufacture protocols.
c) Evaluate the cell count in sample tubes containing sodium citrate anticoagulant and gel separator compared to sample tubes with no additive or gel separator
d) Compare the protocols and their impact on the platelet and cell concentration.
e) Attempt to provide a quality parameter in platelets within each type of protocol.
Results i-PRF resulted in an 86% increase in platelet concentration. Conversely PRP did not fare well as each kit resulted in a decrease of platelets ranging from -16% to -57%
Conclusion This proves that the i-PRF preparation conditions and protocol yielded a significantly higher platelet yield compared to the PRP protocols. This question the future use of PRP and its effectiveness. Is the second-generation blood concentrate i-PRF the better option for clients and clinicians?
Clinical Relevance The variation of platelet and other blood component concentrations between commercial PRP kits may affect clinical treatment outcomes. There is a need for standardization of PRP for clinical use. i-PRF goes some way to offer standardisation and consistency with a simple and effective protocol which offers the highest platelet concentration.
Data To view the study, click the link: https://www.researchgate.net/publication/339618299_Comparative_Study_of_Platelet_Count_Injectable_Platelet_Rich_Fibrin_i-PRF_Compared_to_Platelet_Rich_Plasma_PRP_-Controlled_in_vitro_laboratory_study
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